Agro-forestry programme in India has encouraged the farmers to grow different species of Acacia, Eucalyptus, Albizia and Prosposis in and around their crop fields to fulfil their demands. But recently attention has been focused on allelopathic effect of such trees on germination, growth and yield of the concerned crop. In order to investigate the allelopathic effect of aqueous bark leachate of Acacia nilotica on germination and activities of different enzymes concerned with germination of Phaseolus vulgaris, different concentrations of bark leachate (2-10%) were taken. Pure line seeds of P. vulgaris were germinated in plastic trays (containing cottons) in B.O.D. incubation. Experiments were conducted during 24-144 h for seed germination and 48-144 h for a-amylase and protease activities. Increased concentration of aqueous bark-leachate exhibited a negative correlation with seed germination and biochemical content of germinating seeds. However, a-amylase and protease activities were shown a positive correlation. The bark-leachate of Acacia nilotica contains allelochemicals which might have inhibited the seed germination and reduced the biochemical (carbohydrate and protein) content, whereas a-amylase and protease activities of germinating seeds were increased.
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