Murraya koenigii, or curry leaves (Rutaceae) is indigenous to India and is widely used in Ayurvedic medicine around the world for the treatment and prevention of a wide range of illnesses. Recent research has supported the prospective biological and pharmacological effects of its leaves, such as anticancer, antidiabetic, antioxidant, and anti-inflammatory properties. An improved comprehension of the Chlorophyll (Chl) content of curry leaves will help in relating its therapeutic potential to effective medicine. However, the extraction of pure Chl is challenging due to inefficient extraction and purification techniques. Therefore, we extracted Chl using a solid-liquid extraction method that was dependent on several factors. In this study, the factors affecting the Chl extraction process were optimized by the Response Surface methodology-Central Composite Design (RSM-CCD) approach using Design Expert 11 in relation to solvent concentration, extraction time, and extraction temperature. The responses were obtained after maximizing Chl a and total Chl content. The ideal parameters for the maximum yield of Chl predicted by the software were acetone concentration (100%), extraction time (2 hours), and extraction temperature (50°C). It is anticipated that optimized extraction of Chl will expand its use in various fields.
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