Genetic variability of 16 blackgram (Vigna mungo) genotypes of the family Leguminaceae, one of the important pulses of Odisha, was was studied through RAPD markers. DNA marker analysis revealed an average variation of 42.61% polymorphism in banding patterns of the total score of 176 RAPD amplicons. Cluster analysis of RAPD markers showed that all the genotypes of blackgram studied from Odisha belongs to two distinct clusters i.e. cluster I and II. All the landraces showed narrow genetic diversity except cluster-II (BG-3 of Mahimunda and BG-4 of Berhampur). Among the 10 genotypes of cluster I, BG9, BG10 and BG11 showed close affinity collected from Panthnagar having highest divergence in BG9 which could be used as a potential source of genetically diverse germplasm of blackgram for improved crop breeding for developing YMV resistance.
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