Thin layer chromatography (TLC) profile of Dioscorea pentaphylla L. tuber extracts was performed by using eight different solvent systems. Chloroform: methanol :: 9:1 was found appropriate for fractionation through column chromatography showing maximum number of spot(s) / band(s). Total six fractions were collected and antibacterial activity was examined using disc diffusion assay and agar well diffusion assay. Results indicated that fraction F6 might have an active antibacterial agent (Rf: 0.82). TLC profile and FT-IR analysis of D. pentaphylla tuber extracts can be useful in characterization of different secondary metabolites found in this species.
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