Jatropha curcas L. is a promising source of biodiesel. An efficient protocol was developed for induction of multiple shoots via in vitro culture of shoot tip explants of the Jatropha curcas following inoculation on MS (Murashige and Skoog, 1962) medium supplemented with different concentrations and combinations of cytokinins (BAP/Kinetin) and auxin. The best response achieved on MS medium fortified with BAP 2.0 mg/l + IBA 0.5 mg/l. Maximum rooting was observed on half-strength MS medium with 2.0 mg/l IBA. After successful acclimatization, 70% of the in vitro derived plants survived.
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