• ISSN: 0972-8546
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  • NAAS Rating (Scientific Journals): 3.63

Plant Science Research, Vol. 38 2016, Pages : 76-79

Asit Ray, Biswabhusan Dash and Sanghamitra Nayak

Chromatographic and spectroscopic fingerprint analysis of Hedychium coronarium rhizome extracts through HPLC, GC-MS and FTIR

Hedychium coronarium J. koenig. is an important medicinal and aromatic plant of Zingiberaceae.  Bioactive constituents present in the extract have created a lot of interest because of its  tremendous biological activity. The biggest challenge lies in the authentic identification of the  drug, hence it is of utmost importance to develop spectral and chromatographic fingerprint that  represents pharmacologically active and chemically characteristic components of H. coronarium.  The present study was initiated to carry out chemical fingerprinting using HPLC, FTIR and GCMS.  A total of twelve absorption peaks were present in the IR spectra which can be used to  characterize the extract. This study also attempts to develop HPLC fingerprint of the rhizame  extract. Observation on HPLC spectra shows the presence of twelve distinct peaks. The IR  spectra show the presence of peak at frequencies between ranges from 467.65 to 3327.57 cm-  1. FTIR analysis confirmed the presence of alkane, alkene, alcohol, amines, acid and halogens  in the extract. A total of 21 phytoconstituents was identified in the methanolic extract from the  rhizome extract of the plant by GC-MS. These chemical fingerprinting would be of commercial  significance for quality control and authentication of H. coronarium extracts. 



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