A total of 8 (cloacal and faecal) samples were aseptically collected from different cages of captive exotic birds (Budgerigars, Java sparrow and love birds) from Janpath and Soubhagya nagar area of Bhubaneswar for the isolation, identification and molecular characterization of bacterial flora. All the collected samples were streaked in different selective media for the study of the type of bacteria. Gram staining and biochemical tests were performed for morpho-physiological characterization of the bacteria. This test confirmed the prevalence of E. coli among the isolated bacteria. The antibiogram of the isolated bacteria was performed in which isolated bacteria were found to be highly resistant to cloxacillin, tetracycline and ampicillin and highly sensitive to gentamicin and amikacin. Antibacterial properties of extracts from pulp of wood apple and bark of arjun were evaluated in invitro condition and are found to be ineffective on the isolated bacteria. The presence of virulence gene stx1 in the E. coli was detected using multiplex PCR. Since, many samples were found to contain E. Coli, the handlers must take optimal care during the handling to avoid transmission.
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