PHAs are the biopolymers synthesized by a wide array of bacteria as carbon and energy storage granules. However, its synthesis by sugarcane rhizospheric soil bacteria has gained the utmost attention due to its utilization of a broad spectrum of synthetic or inexpensive substrates. Herein, 20 aerobic bacteria were isolated from the rhizospheric soil of sugarcane using standard bacteriological techniques. Among them, 03 Gram positive bacterial isolates (B1, B2 & B3) were able to accumulate PHAs granule in their cytosol as confirmed from Sudan black B staining. Based on the intensity of staining, bacterial isolate B1 was selected for further study. Under solid-state fermentation (SSF), bacterial isolate B1 was found to produce 1.2 g/l of PHAs using sucrose as carbon source. The bacterial isolate B1 was identified as Bacillus sp. B1 by morpho-physiological characterization. Further, optimization of process parameters, characterization of PHAs and species level identification of the bacterial isolate is highly essential in this regard.
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