Wheat (Triticum aestivum L. cv. Sonalika) seeds were germinated in presence of hexavalent Cr (0.5, 1.0, 2.0, and 4.0 mM) for 24 h and germination percentage, ascorbic acid content, proline content, soluble protein content, activities of antioxidative enzymes like superoxide dismutase (SOD; EC 1.15.1.1), catalase (CAT; EC 1.11.1.6) and peroxidase (POX; EC 1.11.1.7) along with lipid peroxidation level were determined in embryonic tissues. It was found that the activities of SOD, CAT and POX increased with increase of metal in the medium and it was significant (p < 0.05) even at lowest concentration of the metal tested. The level of lipid peroxidation increased significantly (p < 0.05) in the embryonic tissues. Ascorbic acid content decreased whereas proline content increased in the embryonic tissues in response to increase in Cr concentration in the medium. The results indicated the imposition of oxidative stress situations during germination stage by Cr (VI) stress which might be one of the probable reasons behind Cr induced toxicity in germinating seeds.
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