Genetic diversity of ten rice (Oryza sativa L.) varieties of different duration was analyzed through agronomic traits along with RAPD markers. Mid-Early (IR87439, Ciherang Sub1, Lalat), Medium (IR88228, Swarna Sub1, Pratikshya), and Late duration (IR85086, Savitri Sub1, Mahanadi) varieties were tested with a check variety (Swarna) which revealed variation in flowering duration (85 d in Lalat to 110 d in IR85086), plant height and panicle length. 100-grain weight was ranged from 1.92 g in Swarna) to 3.79 g in IR88228. A significant positive correlation of 0.812 was noticed between plant height and leaf area. Plant height, panicle length, flag leaf area have significant positive correlation with 100-grain weight. Out of the 60 RAPD primers, 31 primers produce 280 amplicons (150 to 1960 bp) with a mean 57.24 % polymorphism. Dendrogram obtained from RAPD markers revealed that all the Mid- Early and Medium duration varieties formed Cluster-I except Swarna Sub1 and the rest of the Late duration varieties formed Cluster-II with check variety Swarna. The PCA analysis confirmed that IR88228, IR87439, Ciharang Sub1, and Pratikshya have close genetic similarities as compared to Lalat. Thus, RAPD markers could be used in the identification of varieties and seed certification in rice breeding programs.
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