Plant Science Research, Vol. 42, 2020

Optimization of in vitro root organ culture and co-culture of AM fungi Claroideoglomus claroideum

Arbuscular mycorrhizal (AM) fungi, the obligate symbiont of plant roots require establishment of functional symbiosis with host to complete their life cycle. Among the various methods of mass production of AM inoculum, in vitro method in host root organ culture is a promising method to obtain contamination free high quality inoculum. Besides inoculum production, in vitro method provides opportunity for research in different aspects of host AM interaction. The present study was under taken to optimize the in vitro root organ culture of dicot plants Solanum lycopersicum and Daucus carota, monocot plants Zea mays, Triticum aestivum and Sorghum bicolor for co-culture of AM fungi Claroideoglomus claroideum. Optimization was carried out for root organ proliferation and AM fungi root colonization during co-culture under different cultural conditions such as media consistency and light.