Plant Science Research, Vol. 35, 2013

Callus mediated shoot proliferation from internode explant of Paederia foetida L.

The present study describes the establishment of callus culture and subsequent plant regeneration using in vitro internode explants of Paederia foetida. Four different types of explants were evaluated for callus culture, of which in vitro internode explant was found to be the most suitable. MS basal medium supplemented with 15 different combinations of growth regulators were assessed for callus initiation and proliferation. MS supplemented with 1.5 mg/l N6-benzyladenine (BA) and 0.1 mg/l naphthalene acetic acid (NAA) was found to be the most suitable for establishment of callus culture irrespective of explants used. The highest callus induction (100% and 93.75%) was observed in in vitro internode and in vivo internode explants respectively. The maximum percentage as well as number of shoot regeneration (33.3%; 2) was observed from the callus derived from in vitro internode explants inoculated on MS fortified with 1.0 mg/l BA and 0.5mg/l indole 3-butyric acid (IBA) medium.