Oryza brachyantha (FF), the African wild rice, which is resistant to Yellow stem borer (YSB), was used to develop Monosomic Alien Addition Lines (MAALs) on cultivated rice. A total of 19, 399 BC1F1 spikelets were artificially pollinated with the recurrent parent, out of which 29 BC2F1 hybrids were produced through embryo rescue with the crossability efficiency of 0.14%. Embryos collected at 10- 12 days after pollination (DAP) were grown successfully on ¼ MS basal medium with embryo germination of 35.8%. The plantlets with 4-5 healthy roots were acclimatized by direct transfer method and grown. The hybrids were morphologically characterised into 16 different plant types and each plant type was cytologically analyzed. Hybrids resembling with the morphology of primary trisomics of rice and exhibiting 2n+1 (2n=25) chromosome arrangement were designated as MAALs. A total of 8 MAALs were thus identified representing MAAL- 4 (Sterile), MAAL- 5 (Twisted leaf), MAAL- 7 (Narrow leaf), MAAL- 8 (Rolled leaf), MAAL- 9 (Stout), MAAL- 10 (Erect), MAAL- 11 (Pseudo-normal) and MAAL- 12 (Tall).
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